TtOmp85, a β-Barrel Assembly Protein, Functions by Barrel Augmentation
نویسندگان
چکیده
منابع مشابه
TtOmp85, a β-Barrel Assembly Protein, Functions by Barrel Augmentation
Outer membrane proteins are vital for Gram-negative bacteria and organisms that inherited organelles from them. Proteins from the Omp85/BamA family conduct the insertion of membrane proteins into the outer membrane. We show that an eight-stranded outer membrane β-barrel protein, TtoA, is inserted and folded into liposomes by an Omp85 homologue. Furthermore, we recorded the channel conductance o...
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β-barrel membrane proteins perform important functions in the outer membranes (OMs) of Gram-negative bacteria and of the mitochondria and chloroplasts of eukaryotes. The protein complexes that assemble these proteins in their respective membranes have been identified and shown to contain a component that has been conserved from bacteria to humans. β-barrel proteins are handled differently from ...
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The β-barrel assembly machinery (BAM) complex of Escherichia coli is a multiprotein machine that catalyzes the essential process of assembling outer membrane proteins. The BAM complex consists of five proteins: one membrane protein, BamA, and four lipoproteins, BamB, BamC, BamD, and BamE. Here, we report the first crystal structure of a Bam lipoprotein complex: the essential lipoprotein BamD in...
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The cell envelope protects bacteria from their surroundings. Defects in its integrity or assembly are sensed by signal transduction systems, allowing cells to rapidly adjust. The Rcs phosphorelay responds to outer membrane (OM)- and peptidoglycan-related stress in enterobacteria. We elucidated how the OM lipoprotein RcsF, the upstream Rcs component, senses envelope stress and activates the sign...
متن کاملStructure and Assembly of β-Barrel Membrane Proteins
Integral membrane proteins fall into two major structural classes; they consist of individual or bundled TM -helices, or they form monomeric, dimeric, or trimeric TM -barrels. These folds are dictated by the necessity to form oriented hydrogenbonded secondary structures in the highly ordered apolar environment of the lipid bilayer. No other structural motif has yet been confirmed for membrane p...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2015
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi5011305